Simultaneous determination of primaquine and carboxyprimaquine in plasma using high-performance liquid chromatography with electrochemical detection
Identifieur interne : 002C14 ( Main/Exploration ); précédent : 002C13; suivant : 002C15Simultaneous determination of primaquine and carboxyprimaquine in plasma using high-performance liquid chromatography with electrochemical detection
Auteurs : Robert A. Dean [États-Unis] ; Willis Ochieng [Kenya] ; John Black [États-Unis] ; Sherry F. Queener [États-Unis] ; Marilyn S. Bartlett [États-Unis] ; Natividad G. Dumaual [États-Unis]Source :
- Journal of Chromatography B: Biomedical Sciences and Applications [ 0378-4347 ] ; 1994.
English descriptors
- Teeft :
- Acetonitrile, Acetonitrile extraction, Aids patient, Analytical recovery, Assay, Assay linearity, Assay precision, Calibration curve, Carboxyprimaquine, Carinii, Chromatogr, Chromatographic conditions, Chromatographic method, Concentration controls, Dynamic range, Electrochemical detection, Extraction efficiencies, Extraction efficiency, Hplc system, Human plasma, Human subjects, Indiana university, Maximum plasma concentration, Mobile phase, Multiple drug therapy, Oral administration, Peak heights, Pharmacokinetic analysis, Plasma analysis, Plasma concentration, Plasma samples, Pneumocystis, Pneumocystis carinii, Pneumocystis carinii pneumonia, Pneumocystis pneumonia, Polypropylene tube, Potential interference, Present study, Primaquine, Primaquine administration, Regression line, Retention times, Standard error, Therapeutic drug monitoring, Vertical bars.
Abstract
Abstract: A selective and sensitive high-performance liquid chromatographic method with electrochemical detection is described for the simultaneous quantitation of primaquine and carboxyprimaquine, its primary metabolite, in plasma. After addition of internal standard, plasma was deproteinized by addition of acetonitrile. Nitrogen-dried supernatants, resuspended in mobile phase were analyzed on a C8 reversed-phase column. Limits of detection for primaquine and carboxyprimaquine were 2 and 5 ng/ml with quantitation limits of 5 and 20 ng/ml, respectively. None of 47 tested antimicrobial agents interfered. In contrast to previously reported methods, the assay sensitivity and specificity are sufficient to permit quantitation of primaquine in plasma for pharmacokinetics following low dose (30 mg, base) oral administration of primaquine, typically used in the treatment of malaria and Pneumocystis carinii pneumonia.
Url:
DOI: 10.1016/0378-4347(94)00087-5
Affiliations:
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Dumaual</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, School of Medicine, Indiana University, Indianapolis, IN</wicri:regionArea><placeName><region type="state">Indiana</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Chromatography B: Biomedical Sciences and Applications</title><title level="j" type="abbrev">CHROLD</title><idno type="ISSN">0378-4347</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">655</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="89">89</biblScope><biblScope unit="page" to="96">96</biblScope></imprint><idno type="ISSN">0378-4347</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-4347</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acetonitrile</term><term>Acetonitrile extraction</term><term>Aids patient</term><term>Analytical recovery</term><term>Assay</term><term>Assay linearity</term><term>Assay precision</term><term>Calibration curve</term><term>Carboxyprimaquine</term><term>Carinii</term><term>Chromatogr</term><term>Chromatographic conditions</term><term>Chromatographic method</term><term>Concentration controls</term><term>Dynamic range</term><term>Electrochemical detection</term><term>Extraction efficiencies</term><term>Extraction efficiency</term><term>Hplc system</term><term>Human plasma</term><term>Human subjects</term><term>Indiana university</term><term>Maximum plasma concentration</term><term>Mobile phase</term><term>Multiple drug therapy</term><term>Oral administration</term><term>Peak heights</term><term>Pharmacokinetic analysis</term><term>Plasma analysis</term><term>Plasma concentration</term><term>Plasma samples</term><term>Pneumocystis</term><term>Pneumocystis carinii</term><term>Pneumocystis carinii pneumonia</term><term>Pneumocystis pneumonia</term><term>Polypropylene tube</term><term>Potential interference</term><term>Present study</term><term>Primaquine</term><term>Primaquine administration</term><term>Regression line</term><term>Retention times</term><term>Standard error</term><term>Therapeutic drug monitoring</term><term>Vertical bars</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A selective and sensitive high-performance liquid chromatographic method with electrochemical detection is described for the simultaneous quantitation of primaquine and carboxyprimaquine, its primary metabolite, in plasma. After addition of internal standard, plasma was deproteinized by addition of acetonitrile. Nitrogen-dried supernatants, resuspended in mobile phase were analyzed on a C8 reversed-phase column. Limits of detection for primaquine and carboxyprimaquine were 2 and 5 ng/ml with quantitation limits of 5 and 20 ng/ml, respectively. None of 47 tested antimicrobial agents interfered. In contrast to previously reported methods, the assay sensitivity and specificity are sufficient to permit quantitation of primaquine in plasma for pharmacokinetics following low dose (30 mg, base) oral administration of primaquine, typically used in the treatment of malaria and Pneumocystis carinii pneumonia.</div></front></TEI><affiliations><list><country><li>Kenya</li><li>États-Unis</li></country><region><li>Indiana</li></region></list><tree><country name="États-Unis"><region name="Indiana"><name sortKey="Dean, Robert A" sort="Dean, Robert A" uniqKey="Dean R" first="Robert A." last="Dean">Robert A. 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